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plv cmv myod er t vector  (Addgene inc)


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    Addgene inc plv cmv myod er t vector
    Plv Cmv Myod Er T Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plv+cmv+myod+er+t+vector/pLv-CMV-MyoD-ER(T)+(Plasmid+%2326809)/pmc10951477-92-141-144
    Average 93 stars, based on 7 article reviews
    plv cmv myod er t vector - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Efficient derivation and inducible differentiation of expandable skeletal myogenic cells from human ES and patient-specific iPS cells.
    Article Snippet: KnockOut serum replacement (KSR; Gibco, cat. no. 10828-028) PBS, calcium/magnesium free (Gibco, cat. no. 10010-015) PBS tablets (Sigma, cat. no. P4417) l-Glutamine (Sigma, cat. no. G7513) Penicillin-streptomycin (Sigma, cat. no. P0781) 2-Mercaptoethanol (Gibco, cat. no. 31350-010) Non-essential amino acid solution (Sigma, cat. no. M7145) Human bFGF (Gibco, cat. no. AA 10-155) • • • • • • • • • • • • • • • • • • • Insulin-transferrin-selenium (Gibco, cat. no. 51500-056) Oleic acid (Sigma, cat. no. O1257-10MG) Linoleic acid (Sigma, cat. no. L5900-10MG) Iron sulfate (e.g., Ferinsol, Mead Johnson as detailed in Reagent Setup; alternatively use iron(II) chloride tetrahydrate from Sigma, cat. no. 44939) Sodium ferric gluconate complex (e.g., Ferlixit, Aventis as detailed in the Reagent Setup section; alternatively use iron(III) nitrate nonahydrate from Sigma, cat. no. 254223) Rho-associated protein kinase (ROCK) inhibitor Y27362 (Calbiochem, cat. no. 688000) Growth factor–reduced Matrigel, lactose dehydrogenase elevating virus (LDEV)-free (Corning, cat. no. 356231) Geltrex LDEV-free, hES cell–qualified, reduced growth factor basement membrane matrix (Life Technologies, cat. no. A1413301) EmbryoMax 0.1% (wt/vol) gelatin solution (Merck Millipore, cat. no. ES-006-B) Trypsin (Sigma, cat. no. T3924) 0.5 M EDTA, pH 8.0 (Fisher Scientific, cat. no. 10182903) Paraformaldehyde (TAAB, cat. no. P001) ! cautIon Paraformaldehyde is extremely toxic, and it must be handled with adequate personal protective equipment under a chemical cabinet. .. 4-OH tamoxifen (Sigma, cat. no. H7904) Hexadimethrine bromide (Polybrene; Sigma, cat. no. H9268) pLv-CMV-MyoD-ER(T) vector (Addgene, cat. no. 26809) MyoD-ER(T) integrating lentiviral vector carrying the pLv-CMV-MyoD-ER(T) plasmid ! cautIon Production and work with viral vectors need to be carefully performed under appropriate regulation and must be done in dedicated facilities. ..

    Article Title: An efficient protocol for studying human pluripotent stem cell-derived myotube senescence
    Article Snippet: .. • DMEM/F12 (Gibco, Cat. #11330-032) • GlutaMAXTM-I (Gibco, Cat. #35050-061) • MEM NEAA (Gibco, Cat. #11140-050) • KnockOut TM SR-Muti-Species (Gibco, Cat. #A31815-02) • Penicillin Streptomycin (Gibco, Cat. #15140-122) • 2-Mercaptoethanol (Gibco, Cat. #21985-023) • bFGF (Joint Protein Central, Cat. #BBI-EXP-002) • Fetal Bovine Serum (Gibco, Cat. #26170043) • Dispase (Gibco, Cat. #17105041) • TrypLETM Express Enzyme (Gibco, Cat. #12604-021) • MEMα + GlutaMAXTM-I (Gibco, Cat. #132571-036) • DMEM/High glucose (HyClone, Cat. #SH30243.01) • PlasmocinTM Treatment (InvivoGen, Cat. #ant-mpt) • Phosphate buffered saline (Sigma, Cat. #P4417) • Horse serum (Gibco, Cat. #16050122) • Collagenase-type IV (Gibco, Cat. #17105041) • Polybrene (Sigma, Cat. #107689) • Gelatin from cold water fish skin (Sigma, Cat. #G7041) • 4-hydroxytamoxifen (Sigma, Cat. #H7904) • TGF-β (Humanzyme, Cat. #HST-TB1-1000) • 0.5% growth factor reduced Matrigel (BD Bioscience, Cat. #354230) • Ultra-low attachment 6-well plate (Corning, Cat. #07-200-601) • pLv-CMV-MyoD-ER(T) vector (Addgene, Cat. #26809) • MyoD-ER(T) integrating lentiviral vector carrying the pLv-CMV-MyoD-ER(T) plasmid • Anti CD73-PE antibody (BD Bioscience, Cat. #550257) • Anti CD90-FITC antibody (BD Bioscience, Cat. #555595) • Anti CD105-APC antibody (BD Bioscience, Cat. #17105742) • Hoechst 33342 (Invitrogen, Cat. #H3570) • Anti MyHC antibody (DSHB, Cat. #MF20) .. • Centrifuge (Thermo Fisher Scientific, Cat. #MicroCL 21R) • Flow cytometer (BD, FACSAria III) • Cell incubator, 5% CO 2 , 3%–5% O 2 (Thermofosher, 150i) • Cell incubator, 5% CO 2 , 21% O 2 (Thermofosher, 150i)

    Knock-Out:

    Article Title: An efficient protocol for studying human pluripotent stem cell-derived myotube senescence
    Article Snippet: .. • DMEM/F12 (Gibco, Cat. #11330-032) • GlutaMAXTM-I (Gibco, Cat. #35050-061) • MEM NEAA (Gibco, Cat. #11140-050) • KnockOut TM SR-Muti-Species (Gibco, Cat. #A31815-02) • Penicillin Streptomycin (Gibco, Cat. #15140-122) • 2-Mercaptoethanol (Gibco, Cat. #21985-023) • bFGF (Joint Protein Central, Cat. #BBI-EXP-002) • Fetal Bovine Serum (Gibco, Cat. #26170043) • Dispase (Gibco, Cat. #17105041) • TrypLETM Express Enzyme (Gibco, Cat. #12604-021) • MEMα + GlutaMAXTM-I (Gibco, Cat. #132571-036) • DMEM/High glucose (HyClone, Cat. #SH30243.01) • PlasmocinTM Treatment (InvivoGen, Cat. #ant-mpt) • Phosphate buffered saline (Sigma, Cat. #P4417) • Horse serum (Gibco, Cat. #16050122) • Collagenase-type IV (Gibco, Cat. #17105041) • Polybrene (Sigma, Cat. #107689) • Gelatin from cold water fish skin (Sigma, Cat. #G7041) • 4-hydroxytamoxifen (Sigma, Cat. #H7904) • TGF-β (Humanzyme, Cat. #HST-TB1-1000) • 0.5% growth factor reduced Matrigel (BD Bioscience, Cat. #354230) • Ultra-low attachment 6-well plate (Corning, Cat. #07-200-601) • pLv-CMV-MyoD-ER(T) vector (Addgene, Cat. #26809) • MyoD-ER(T) integrating lentiviral vector carrying the pLv-CMV-MyoD-ER(T) plasmid • Anti CD73-PE antibody (BD Bioscience, Cat. #550257) • Anti CD90-FITC antibody (BD Bioscience, Cat. #555595) • Anti CD105-APC antibody (BD Bioscience, Cat. #17105742) • Hoechst 33342 (Invitrogen, Cat. #H3570) • Anti MyHC antibody (DSHB, Cat. #MF20) .. • Centrifuge (Thermo Fisher Scientific, Cat. #MicroCL 21R) • Flow cytometer (BD, FACSAria III) • Cell incubator, 5% CO 2 , 3%–5% O 2 (Thermofosher, 150i) • Cell incubator, 5% CO 2 , 21% O 2 (Thermofosher, 150i)

    Saline:

    Article Title: An efficient protocol for studying human pluripotent stem cell-derived myotube senescence
    Article Snippet: .. • DMEM/F12 (Gibco, Cat. #11330-032) • GlutaMAXTM-I (Gibco, Cat. #35050-061) • MEM NEAA (Gibco, Cat. #11140-050) • KnockOut TM SR-Muti-Species (Gibco, Cat. #A31815-02) • Penicillin Streptomycin (Gibco, Cat. #15140-122) • 2-Mercaptoethanol (Gibco, Cat. #21985-023) • bFGF (Joint Protein Central, Cat. #BBI-EXP-002) • Fetal Bovine Serum (Gibco, Cat. #26170043) • Dispase (Gibco, Cat. #17105041) • TrypLETM Express Enzyme (Gibco, Cat. #12604-021) • MEMα + GlutaMAXTM-I (Gibco, Cat. #132571-036) • DMEM/High glucose (HyClone, Cat. #SH30243.01) • PlasmocinTM Treatment (InvivoGen, Cat. #ant-mpt) • Phosphate buffered saline (Sigma, Cat. #P4417) • Horse serum (Gibco, Cat. #16050122) • Collagenase-type IV (Gibco, Cat. #17105041) • Polybrene (Sigma, Cat. #107689) • Gelatin from cold water fish skin (Sigma, Cat. #G7041) • 4-hydroxytamoxifen (Sigma, Cat. #H7904) • TGF-β (Humanzyme, Cat. #HST-TB1-1000) • 0.5% growth factor reduced Matrigel (BD Bioscience, Cat. #354230) • Ultra-low attachment 6-well plate (Corning, Cat. #07-200-601) • pLv-CMV-MyoD-ER(T) vector (Addgene, Cat. #26809) • MyoD-ER(T) integrating lentiviral vector carrying the pLv-CMV-MyoD-ER(T) plasmid • Anti CD73-PE antibody (BD Bioscience, Cat. #550257) • Anti CD90-FITC antibody (BD Bioscience, Cat. #555595) • Anti CD105-APC antibody (BD Bioscience, Cat. #17105742) • Hoechst 33342 (Invitrogen, Cat. #H3570) • Anti MyHC antibody (DSHB, Cat. #MF20) .. • Centrifuge (Thermo Fisher Scientific, Cat. #MicroCL 21R) • Flow cytometer (BD, FACSAria III) • Cell incubator, 5% CO 2 , 3%–5% O 2 (Thermofosher, 150i) • Cell incubator, 5% CO 2 , 21% O 2 (Thermofosher, 150i)



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